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fitcvicia villosa agglutinin  (Vector Laboratories)


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    Structured Review

    Vector Laboratories fitcvicia villosa agglutinin
    Fitcvicia Villosa Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 79 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/FL-1231-2/Fluorescein+labeled+Vicia+Villosa+Lectin+(VVL%2C+VVA)/pm42878060-189-9-13
    Average 95 stars, based on 79 article reviews
    fitcvicia villosa agglutinin - by Bioz Stars, 2026-11
    95/100 stars

    Images

    Related Articles

    Isolation:

    Article Title: Sialylated CD43 forms a glyco-immune barrier that restrains antileukemic immunity.
    Article Snippet: INTRODUCTION: Macrophages in the tumor microenvironment exert antitumorigenic effects through phagocytosis and/or direct tumoricidal activity.. Phagocytosis of tumor cells occurs through both antibodydependent cellular phagocytosis (ADCP) and antibodyindependent cellular phagocytosis (AICP) mechanisms.. Despite the strong evidence that macrophages can mediate tumor control in acute myeloid leukemia (AML) and other diseases, therapeutic agents that enhance macrophage phagocytosis, including antiCD47 neutralizing antibodies, have not led to improved clinical outcomes.

    Staining:

    Article Title: Sialylated CD43 forms a glyco-immune barrier that restrains antileukemic immunity.
    Article Snippet: INTRODUCTION: Macrophages in the tumor microenvironment exert antitumorigenic effects through phagocytosis and/or direct tumoricidal activity.. Phagocytosis of tumor cells occurs through both antibodydependent cellular phagocytosis (ADCP) and antibodyindependent cellular phagocytosis (AICP) mechanisms.. Despite the strong evidence that macrophages can mediate tumor control in acute myeloid leukemia (AML) and other diseases, therapeutic agents that enhance macrophage phagocytosis, including antiCD47 neutralizing antibodies, have not led to improved clinical outcomes.

    Article Title: Disrupted O-GalNAc glycosylation as a mechanism and biomarker of SLC35A2 -associated epilepsy
    Article Snippet: After fixation, brains were incubated in 30% sucrose solution and sections were cut on a freezing microtome (Leica #SM2010R) at 30-μm thickness. .. Sections were blocked, stained, and washed as above for nitrocellulose blots with the following FITC lectins (Vector Labs, VVA #FL-1231-2, PNA #FL-1071-5, ConA #FL-10011-5, AAL #FL-1391-1, and RCA #FL1081-1) at 25 mg/mL, biotinylated WFA (#B-1355-2) at 25 mg/mL, or primary antibody for myelin basic protein (Cell Signaling #E9P7U) at 1:100 overnight at 4°C. .. Sections were washed with TBS-T 3 times as above, and secondary streptavidin AF568 (Invitrogen #S11226) or anti-mouse AF555 (Jackson Immuno #717-565-150) at 1:1,000 for 1 hour at room temperature followed by 3 additional washes, with the second wash containing 1:1,000 Hoechst-33342 (Thermo #62249).

    other:


    Whole Genome Amplification:

    Article Title: Graph attention network-driven single-cell omics analysis reveals how B4GALNT1-mediated glycosylation remodelling of the ferroptosis-pyroptosis network promotes drug resistance in hepatocellular carcinoma.
    Article Snippet: .. Fluorescently labelled lectins were used in the experiment, including FITCvicia villosa agglutinin (VVA; Vector Laboratories, FL-1231, USA), FITC-soybean agglutinin (SBA) (Vector Laboratories, FL-1011, USA), Alexa Fluor 488-labelled wheat germ agglutinin (WGA; ThermoFisher Scientific,W11261, USA), and FITC-Ricinus Communis Agglutinin I (RCA-I) (Vector Laboratories, FL-1081, USA). ..

    Injection:

    Article Title: Early postsynaptic instability and acetylcholine receptor compartmentalization precede neuromuscular synapse dismantling
    Article Snippet: After several washes with PBT, samples were incubated with the secondary antibodies (Cy5, 1:250; Donkey H + L, Jackson Immunoresearch Laboratories) overnight at 4 °C, then washed for 2 h with PBT and 15 min with PBS before being mounted between two coverslips in DAKO fluorescence medium. .. To label the location of NMJs before denervation, fluorescein-conjugated Vicia villosa Lectin (VVA, Vector Laboratories) was injected subcutaneously in vivo (20 μg/mL in PBS) on the top of denervated, or contralateral LAL muscles previously labeled with Alexa647-BTX (BTX1, pseudo-colored green) 6 days before. .. To label the location of NMJs before denervation, fluorescein-conjugated Vicia villosa Lectin (VVA, Vector Laboratories) was injected subcutaneously in vivo (20 μg/mL in PBS) on the top of denervated, or contralateral LAL muscles previously labeled with Alexa647-BTX (BTX1, pseudo-colored green) 6 days before.

    In Vivo:

    Article Title: Early postsynaptic instability and acetylcholine receptor compartmentalization precede neuromuscular synapse dismantling
    Article Snippet: After several washes with PBT, samples were incubated with the secondary antibodies (Cy5, 1:250; Donkey H + L, Jackson Immunoresearch Laboratories) overnight at 4 °C, then washed for 2 h with PBT and 15 min with PBS before being mounted between two coverslips in DAKO fluorescence medium. .. To label the location of NMJs before denervation, fluorescein-conjugated Vicia villosa Lectin (VVA, Vector Laboratories) was injected subcutaneously in vivo (20 μg/mL in PBS) on the top of denervated, or contralateral LAL muscles previously labeled with Alexa647-BTX (BTX1, pseudo-colored green) 6 days before. .. To label the location of NMJs before denervation, fluorescein-conjugated Vicia villosa Lectin (VVA, Vector Laboratories) was injected subcutaneously in vivo (20 μg/mL in PBS) on the top of denervated, or contralateral LAL muscles previously labeled with Alexa647-BTX (BTX1, pseudo-colored green) 6 days before.

    Muscles:

    Article Title: Early postsynaptic instability and acetylcholine receptor compartmentalization precede neuromuscular synapse dismantling
    Article Snippet: After several washes with PBT, samples were incubated with the secondary antibodies (Cy5, 1:250; Donkey H + L, Jackson Immunoresearch Laboratories) overnight at 4 °C, then washed for 2 h with PBT and 15 min with PBS before being mounted between two coverslips in DAKO fluorescence medium. .. To label the location of NMJs before denervation, fluorescein-conjugated Vicia villosa Lectin (VVA, Vector Laboratories) was injected subcutaneously in vivo (20 μg/mL in PBS) on the top of denervated, or contralateral LAL muscles previously labeled with Alexa647-BTX (BTX1, pseudo-colored green) 6 days before. .. To label the location of NMJs before denervation, fluorescein-conjugated Vicia villosa Lectin (VVA, Vector Laboratories) was injected subcutaneously in vivo (20 μg/mL in PBS) on the top of denervated, or contralateral LAL muscles previously labeled with Alexa647-BTX (BTX1, pseudo-colored green) 6 days before.

    Labeling:

    Article Title: Early postsynaptic instability and acetylcholine receptor compartmentalization precede neuromuscular synapse dismantling
    Article Snippet: After several washes with PBT, samples were incubated with the secondary antibodies (Cy5, 1:250; Donkey H + L, Jackson Immunoresearch Laboratories) overnight at 4 °C, then washed for 2 h with PBT and 15 min with PBS before being mounted between two coverslips in DAKO fluorescence medium. .. To label the location of NMJs before denervation, fluorescein-conjugated Vicia villosa Lectin (VVA, Vector Laboratories) was injected subcutaneously in vivo (20 μg/mL in PBS) on the top of denervated, or contralateral LAL muscles previously labeled with Alexa647-BTX (BTX1, pseudo-colored green) 6 days before. .. To label the location of NMJs before denervation, fluorescein-conjugated Vicia villosa Lectin (VVA, Vector Laboratories) was injected subcutaneously in vivo (20 μg/mL in PBS) on the top of denervated, or contralateral LAL muscles previously labeled with Alexa647-BTX (BTX1, pseudo-colored green) 6 days before.



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    (A) Dynamics of CP2-HA secretion during invasion and early growth of C. parvum as revealed by immunofluorescence microscopy. HCT-8 cells were infected with CP2-HA sporozoites, fixed at 15 min, 30 min, 1 h and 2 h post-infection (hpi), and stained with rabbit anti-HA (red), anti- Cp that recognizes whole parasites (green), and Hoechst (blue). Scale bars, 2 μm. (B-E) Immunofluorescence localization of CP2-HA in trophozoites and meronts in HCT-8 cultures. HCT-8 cells were fixed at 24 hpi and stained with rabbit anti-HA (red), <t>Vicia</t> <t>villosa</t> <t>lectin</t> <t>(VVL,</t> green) and Hoechst (blue). Both the top (B and C) and side (D and E) views of the same parasites are shown. Scale bars, 2 μm. (F-G) Ultrastructural localization of CP2-HA in the feeder organelle (F), trophozoite (G), and meront (H) by immunoelectron microscopy of the ileal tissue from mice infected with CP2-HA, using a rabbit anti-HA antibody and 10-nm colloidal gold-conjugated goat-anti-rabbit IgG. Black arrowheads indicate the distribution of gold particles. N, nucleus; SG, small granules. Scale bars, 500 nm.
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    (A) Dynamics of CP2-HA secretion during invasion and early growth of C. parvum as revealed by immunofluorescence microscopy. HCT-8 cells were infected with CP2-HA sporozoites, fixed at 15 min, 30 min, 1 h and 2 h post-infection (hpi), and stained with rabbit anti-HA (red), anti- Cp that recognizes whole parasites (green), and Hoechst (blue). Scale bars, 2 μm. (B-E) Immunofluorescence localization of CP2-HA in trophozoites and meronts in HCT-8 cultures. HCT-8 cells were fixed at 24 hpi and stained with rabbit anti-HA (red), <t>Vicia</t> <t>villosa</t> <t>lectin</t> <t>(VVL,</t> green) and Hoechst (blue). Both the top (B and C) and side (D and E) views of the same parasites are shown. Scale bars, 2 μm. (F-G) Ultrastructural localization of CP2-HA in the feeder organelle (F), trophozoite (G), and meront (H) by immunoelectron microscopy of the ileal tissue from mice infected with CP2-HA, using a rabbit anti-HA antibody and 10-nm colloidal gold-conjugated goat-anti-rabbit IgG. Black arrowheads indicate the distribution of gold particles. N, nucleus; SG, small granules. Scale bars, 500 nm.
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    Image Search Results


    (A) Dynamics of CP2-HA secretion during invasion and early growth of C. parvum as revealed by immunofluorescence microscopy. HCT-8 cells were infected with CP2-HA sporozoites, fixed at 15 min, 30 min, 1 h and 2 h post-infection (hpi), and stained with rabbit anti-HA (red), anti- Cp that recognizes whole parasites (green), and Hoechst (blue). Scale bars, 2 μm. (B-E) Immunofluorescence localization of CP2-HA in trophozoites and meronts in HCT-8 cultures. HCT-8 cells were fixed at 24 hpi and stained with rabbit anti-HA (red), Vicia villosa lectin (VVL, green) and Hoechst (blue). Both the top (B and C) and side (D and E) views of the same parasites are shown. Scale bars, 2 μm. (F-G) Ultrastructural localization of CP2-HA in the feeder organelle (F), trophozoite (G), and meront (H) by immunoelectron microscopy of the ileal tissue from mice infected with CP2-HA, using a rabbit anti-HA antibody and 10-nm colloidal gold-conjugated goat-anti-rabbit IgG. Black arrowheads indicate the distribution of gold particles. N, nucleus; SG, small granules. Scale bars, 500 nm.

    Journal: PLOS Pathogens

    Article Title: Small granule protein CP2 of Cryptosporidium translocates to the parasite-host interface during invasion and localizes to parasitophorous vacuole membrane in association with other secretory proteins

    doi: 10.1371/journal.ppat.1013847

    Figure Lengend Snippet: (A) Dynamics of CP2-HA secretion during invasion and early growth of C. parvum as revealed by immunofluorescence microscopy. HCT-8 cells were infected with CP2-HA sporozoites, fixed at 15 min, 30 min, 1 h and 2 h post-infection (hpi), and stained with rabbit anti-HA (red), anti- Cp that recognizes whole parasites (green), and Hoechst (blue). Scale bars, 2 μm. (B-E) Immunofluorescence localization of CP2-HA in trophozoites and meronts in HCT-8 cultures. HCT-8 cells were fixed at 24 hpi and stained with rabbit anti-HA (red), Vicia villosa lectin (VVL, green) and Hoechst (blue). Both the top (B and C) and side (D and E) views of the same parasites are shown. Scale bars, 2 μm. (F-G) Ultrastructural localization of CP2-HA in the feeder organelle (F), trophozoite (G), and meront (H) by immunoelectron microscopy of the ileal tissue from mice infected with CP2-HA, using a rabbit anti-HA antibody and 10-nm colloidal gold-conjugated goat-anti-rabbit IgG. Black arrowheads indicate the distribution of gold particles. N, nucleus; SG, small granules. Scale bars, 500 nm.

    Article Snippet: After three washes with PBS, the cells were incubated at 37°C for 60 min with Alexa Fluor-conjugated secondary antibodies (Thermo Fisher Scientific) diluted 1:500 in 1% BSA-PBS, fluorescein-conjugated Vicia villosa lectin (VVL) (Vector; Newark, NJ, USA) diluted 1:500, or fluorescein-conjugated Sporo-Glo polyclonal rat IgG antibody diluted 1:20 (Waterborne, Inc., New Orleans, LA, USA).

    Techniques: Immunofluorescence, Microscopy, Infection, Staining, Immuno-Electron Microscopy

    (A-C) Immunofluorescence localization of SG3-HA (cgd6_5400-HA), DG7-HA (cgd6_5420-HA), and DG8-HA (cgd8_5300-HA) in sporozoites. The schematics show the domain structure of SG3, DG7 and DG8, including the putative signal peptide (orange), predicted repeat domains (light blue), and the location of the 3HA tag. Sporozoites from the transgenic lines were fixed and stained with rabbit anti-HA (red), anti-EF1α (green), and Hoechst. Scale bars, 2 μm. (D-F) U-ExM of SG3-HA, DG7-HA and DG8-HA in sporozoites, after expansion in gel and staining with rat anti-HA (green), NHS ester (gey), and Hoechst (blue). Scale bars, 5 μm. (G, I, and K) Immunofluorescence localization of SG3, DG7 and DG8 in trophozoites and meronts in HCT-8 cells at 24 hpi, after staining with rabbit anti-HA (red), Vicia villosa lectin (VVL, green), and Hoechst (blue). Scale bars, 2 μm. (H, J, and L) Co-localization of SG3-HA, DG7-HA and DG8-HA with CP2 in trophozoites and meronts in HCT-8 cells at 24 hpi, after staining with rabbit anti-HA (green), anti-CP2 (red), and Hoechst (blue). Scale bars, 2 μm. (M and N) Ultrastructural localization of SG3-HA and DG8-HA in trophozoites and meronts by immunoelectron microscopy of the ileum from infected mice, using a rabbit anti-HA antibody and a 12-nm colloidal gold-conjugated goat-anti-rabbit IgG antibody. Scale bars, 200 nm.

    Journal: PLOS Pathogens

    Article Title: Small granule protein CP2 of Cryptosporidium translocates to the parasite-host interface during invasion and localizes to parasitophorous vacuole membrane in association with other secretory proteins

    doi: 10.1371/journal.ppat.1013847

    Figure Lengend Snippet: (A-C) Immunofluorescence localization of SG3-HA (cgd6_5400-HA), DG7-HA (cgd6_5420-HA), and DG8-HA (cgd8_5300-HA) in sporozoites. The schematics show the domain structure of SG3, DG7 and DG8, including the putative signal peptide (orange), predicted repeat domains (light blue), and the location of the 3HA tag. Sporozoites from the transgenic lines were fixed and stained with rabbit anti-HA (red), anti-EF1α (green), and Hoechst. Scale bars, 2 μm. (D-F) U-ExM of SG3-HA, DG7-HA and DG8-HA in sporozoites, after expansion in gel and staining with rat anti-HA (green), NHS ester (gey), and Hoechst (blue). Scale bars, 5 μm. (G, I, and K) Immunofluorescence localization of SG3, DG7 and DG8 in trophozoites and meronts in HCT-8 cells at 24 hpi, after staining with rabbit anti-HA (red), Vicia villosa lectin (VVL, green), and Hoechst (blue). Scale bars, 2 μm. (H, J, and L) Co-localization of SG3-HA, DG7-HA and DG8-HA with CP2 in trophozoites and meronts in HCT-8 cells at 24 hpi, after staining with rabbit anti-HA (green), anti-CP2 (red), and Hoechst (blue). Scale bars, 2 μm. (M and N) Ultrastructural localization of SG3-HA and DG8-HA in trophozoites and meronts by immunoelectron microscopy of the ileum from infected mice, using a rabbit anti-HA antibody and a 12-nm colloidal gold-conjugated goat-anti-rabbit IgG antibody. Scale bars, 200 nm.

    Article Snippet: After three washes with PBS, the cells were incubated at 37°C for 60 min with Alexa Fluor-conjugated secondary antibodies (Thermo Fisher Scientific) diluted 1:500 in 1% BSA-PBS, fluorescein-conjugated Vicia villosa lectin (VVL) (Vector; Newark, NJ, USA) diluted 1:500, or fluorescein-conjugated Sporo-Glo polyclonal rat IgG antibody diluted 1:20 (Waterborne, Inc., New Orleans, LA, USA).

    Techniques: Immunofluorescence, Transgenic Assay, Staining, Immuno-Electron Microscopy, Infection